Recombinant Human NOTCH4 Protein (Arg24-Glu549), C-Fc-tagged
Product Description
Cat
IMP-9995
Official Symbol
NOTCH4
Product Overview
Recombinant human Notch-4 protein (Arg24-Glu549) with a human IgG1 (Pro100-Lys330) Fc tag was eexpressed in Trichoplusia ni.
Description
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Expression System
T. ni
Species
Human
Tag
C-Fc
Predicted N Terminal
Arg24
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 82 kDa
SDS-PAGE: 94-116 kDa, reducing conditions
Protein length
Arg24-Glu549
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human DLL4 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Notch-4 Human Fc Chimera that produces 50% of the optimal binding response is 0.5-2.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human DLL4 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Notch-4 Human Fc Chimera that produces 50% of the optimal binding response is 0.5-2.5 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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