IMP-4859

NPM1

Recombinant human NPM1 isoform 1 (P06748-1) N-terminal segment (Met 9-Leu 158) was expressed, with a polyhistide tag at the N-terminus.

Nucleophosmin 1 (NPM1), also known as nucleolar phosphoprotein B23 or numatrin, is a member of the nucleoplasmin family. Nucleophosmin (NPM) is a nucleolar phosphoprotein that plays multiple roles in ribosome assembly and transport, cytoplasmic-nuclear trafficking, centrosome duplication, and regulation of p53. The NPM1 gene is frequently involved in chromosomal translocation, mutation, and deletion. Mutations of the NPM1 gene leading to the expression of a cytoplasmic mutant protein, NPMc+, are the most frequent genetic abnormalities found in acute myeloid leukemias. Acute myeloid leukemia (AML) with mutated NPM1 have distinct characteristics, including a significant association with a normal karyotype, the involvement of different hematopoietic lineages, a specific gene-expression profile, and clinically, a better response to induction therapy, and a favorable prognosis. Also, NPM1 is a crucial gene to consider in the context of the genetics and biology of cancer. NPM1 is frequently overexpressed, mutated, rearranged, and deleted in human cancer. Traditionally regarded as a tumor marker and a putative proto-oncogene, it has now also been attributed with tumor-suppressor functions.

E. coli

Human

N-His

Met

Lyophilized from sterile PBS, pH 6.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human NPM1 consisting of 161 amino acids and has a calculated molecular mass of 17.8 kDa. It migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.

Met9-Leu158

Please contact us for more information.

> 90 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.