Recombinant Human NRP1 Protein, C-Avi-His tag, Biotinylated

Product Description

Cat

IMP-980

Official Symbol

NRP1

Product Overview

Biotinylated Recombinant Human NRP1(AAH07533,1)(Phe22-Lys644) Protein, fused with a Avi, 6*His tag at the C-terminus, is expressed from HEK293 cells.

Expression System

HEK293

Species

Human

Tag

C-Avi-His tag

Form

Lyophilized from sterile PBS, pH 7.4.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 72.5kDa, and migrates as 85-100kDa under reducing conditions.

Protein length

Phe22-Lys644

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Notes

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Anti-Human NRP1 mAb at 2μg/ml (100 μl/well) can bind Human Neuropilin-1-Avi-His. The ED50 of Human Neuropilin-1-Avi-His is 9.33 ng/ml.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry