Recombinant Human NT5E protein, C-Fc Tag

Product Description

Cat

IMP-595

Official Symbol

NT5E

Product Overview

Recombinant Human NT5E(NP_001191742.1)(Trp27-Lys547) is expressed in HEK293, fused with a Fc tag at the C-terminus.

Description

CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.

Molecular Mass

85-100kDa, reducing conditions.

Protein length

Trp27-Lys547

Endotoxin

Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Storage

Store at ≤-70°C, stable for 6 months after receipt.

Concentration

Please refer to product label for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human CD73-Fc at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb. Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CD73 mAb is 0.31 ug/ml.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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