Recombinant Human NT5E protein, C-His tag

Product Description

Cat

IMP-744

Official Symbol

NT5E

Product Overview

Recombinant Human NT5E(P21589)(Trp27-Lys547) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-His tag

Form

Liquid in sterile 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.

Molecular Mass

The protein has a calculated MW of 58.8kDa, and migrates as 62kDa under reducing conditions.

Protein length

Trp27-Lys547

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Concentration

Please refer to the COA for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human CD73-His at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb. The ED50 of Anti-Human CD73 mAb is 7.8 ng/ml.

Bioactivity-BLI 1

Loaded Anti-Human CD73 mAb-Fc on Protein A Biosensor, can bind Human CD73-His with an affinity constant of 0.60 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry