IMP-5019

NTRK3

Recombinant extracellular domain (Met1-Asp428) of human TrkC (NP_001007157.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NT-3 growth factor receptor is also known as neurotrophic tyrosine kinase receptor type 3 or TrkC tyrosine kinase or Trk-C receptor, is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC/NTRK3 is widely expressed in the developing and adult nervous system. In later embryonic development, TrkC/NTRK3 is expressed in various structures of the CNS including the caudate-putamen, septal nuclei, cerebellum, and brainstem. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. In the PNS, The trkC hybridization appears to correlate, both temporally and spatially, with the outgrowth of axons toward their peripheral targets. TrkC/NTRK3 is widely expressed in the three identified branches of the mammalian nervous system and appears to correlate with the expression of NT-3, its cognate ligand. The apparent colocalization of trkC transcripts with NT-3 raises the possibility this neurotrophin exerts its trophic effects by a paracrine and/or autocrine mechanism. Signaling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in the TrkC encoding gene have been associated with medulloblastomas, secretory breast carcinomas, and other cancers.

HEK293

Human

C-His&C-hFc

Cys 32

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human TrkC/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. It consists of 644 amino acids and has a calculated molecular mass of 72.5 kDa. As a result of glycosylation, rh TrkC/Fc monomer migrates as an approximately 110-120 kDa protein in SDS-PAGE under reducing conditions.

Met1-Asp428

< 1.0 EU/μg of the protein as determined by the LAL method

> 98 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Immobilized human TrKc-Fch at 10 μg/mL (100 μL/well) can bind biotinylated human NT3, The EC50 of biotinylated human NT3 is 23.4-54.6 ng/mL.