Recombinant Human PDCD1 protein, C-Fc-Avi Tag, Biotinylated

Product Description

Cat

IMP-627

Official Symbol

PDCD1

Product Overview

Biotinylated Recombinant Human PDCD1 protein(NP_005009.2)(Leu 25-Gln 167), fused to C-Fc-Avi Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-Fc-Avi Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 44.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.
>95% as determined by SEC-MALS.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

SEC-MALS 1

Bioactivity-ELISA 1

Immobilized Human PD-L1, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag with a linear range of 0.02-0.32 μg/mL.

Bioactivity-ELISA 2

Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag with a linear range of 0.2-6 ng/mL.

Bioactivity-ELISA 3

Immobilized Biotinylated Human PD-1, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Mouse PD-L2, His Tag with a linear range of 20-156 ng/mL.

Bioactivity-ELISA 4

Serial dilutions of nivolumab were added into Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified): Biotinylated Human PD-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.3789 μg/mL.

Bioactivity-BLI 1

Loaded Biotinylated Human PD-1, Fc,Avitag on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) with an affinity constant of 2.5 μM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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