Immobilized Human PD-L1, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag with a linear range of 0.02-0.32 μg/mL.
Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag with a linear range of 0.2-6 ng/mL.
Immobilized Biotinylated Human PD-1, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Mouse PD-L2, His Tag with a linear range of 20-156 ng/mL.
Serial dilutions of nivolumab were added into Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified): Biotinylated Human PD-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.3789 μg/mL.
Loaded Biotinylated Human PD-1, Fc,Avitag on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) with an affinity constant of 2.5 μM as determined in BLI assay.