Recombinant Human PDCD1 protein, C-Fc-Avi Tag, Biotinylated
Product Description
Cat
IMP-627
Official Symbol
PDCD1
Product Overview
Biotinylated Recombinant Human PDCD1 protein(NP_005009.2)(Leu 25-Gln 167), fused to C-Fc-Avi Tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-Fc-Avi Tag, Biotinylated
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a calculated MW of 44.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Leu 25-Gln 167
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.
>95% as determined by SEC-MALS.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

SEC-MALS 1
SEC-MALS

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human PD-L1, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag with a linear range of 0.02-0.32 μg/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag with a linear range of 0.2-6 ng/mL.

Bioactivity-ELISA 3
Bioactivity-ELISA

Immobilized Biotinylated Human PD-1, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Mouse PD-L2, His Tag with a linear range of 20-156 ng/mL.

Bioactivity-ELISA 4
Bioactivity-ELISA

Serial dilutions of nivolumab were added into Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified): Biotinylated Human PD-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.3789 μg/mL.

Bioactivity-BLI 1

Loaded Biotinylated Human PD-1, Fc,Avitag on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) with an affinity constant of 2.5 μM as determined in BLI assay.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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