Recombinant Human PDCD1 protein, C-Fc-His Tag, PE-Labeled

Product Description

Cat

IMP-628

Official Symbol

PDCD1

Product Overview

PE-Labeled Recombinant Human PDCD1 protein(NP_005009.2)(Leu 25-Gln 167), fused to C-Fc-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-Fc-His Tag, PE-Labeled

Form

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 44.7 kDa.

Protein length

Leu 25-Gln 167

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

Bioactivity-FACS 1

Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) can bind to 293T cells overexpressing human PD-L1. 1 μl stock solution per million cells.

Bioactivity-FACS 2

FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc,His Tag to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-human PD-L1 antibody. The IC50 is 0.29 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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