Recombinant Human PDCD1 protein, C-Fc-His Tag, PE-Labeled

Product Description

Cat

IMP-628

Official Symbol

PDCD1

Product Overview

PE-Labeled Recombinant Human PDCD1 protein(NP_005009.2)(Leu 25-Gln 167), fused to C-Fc-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-Fc-His Tag, PE-Labeled

Form

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 44.7 kDa.

Protein length

Leu 25-Gln 167

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

Bioactivity-FACS 1

Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) can bind to 293T cells overexpressing human PD-L1. 1 μl stock solution per million cells.

Bioactivity-FACS 2

FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc,His Tag to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-human PD-L1 antibody. The IC50 is 0.29 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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