Recombinant Human PDCD1 protein, C-Fc Tag

Product Description

Cat

IMP-052

Official Symbol

PDCD1

Product Overview

Recombinant Human PDCD1 protein(AA Leu 25-Gln 167)(Accession # NP_005009.2) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 42.1 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-1, Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-1, Fc Tag, low endotoxin was more than 90% and the molecular weight of this protein is around 130-150 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) at 1 ug/mL (100 uL/well) on streptavidin precoated (0.2 ug/well) plate, can bind Human PD-1, Fc Tag, low endotoxin with a linear range of 0.1-3 ng/mL.

Bioactivity-ELISA 2

Immobilized Human PD-1, Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Biotinylated Human PD-L2, Fc,Avitag with a linear range of 0.5-16 ng/mL.

Bioactivity-ELISA 3

Immobilized Biotinylated Human PD-L2, Avitag,His Tag (recommended for biopanning) at 1 ug/mL (100 uL/well) on streptavidin precoated (0.2 ug/well) plate, can bind Human PD-1, Fc Tag, low endotoxin with a linear range of 0.1-1 ng/mL.

Bioactivity-ELISA 4

Serial dilutions of nivolumab were added into Human PD-1, Fc Tag, low endotoxin: Biotinylated Human PD-L1, Fc,Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 ug/mL.

Bioactivity-BLI 1

Loaded Human PD-1, Fc Tag, low endotoxin on ProteinA Biosensor, can bind Human PD-L1, His Tag (HPLC & BLI verified) with an affinity constant of 5.3 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-1, Fc Tag, low endotoxin on Protein A Biosensor, can bind Human PD-L2, His Tag (SPR verified) with an affinity constant of 0.45 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that Human PD-1, Fc Tag, low endotoxin can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 ug/mL.

Bioactivity-SPR 1

Human PD-1, Fc Tag, low endotoxin (HPLC-verified) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (HPLC-verified) with an affinity constant of 3.6 uM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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