Recombinant Human PDCD1 protein, C-Fc Tag

Product Description

Cat

IMP-052

Official Symbol

PDCD1

Product Overview

Recombinant Human PDCD1 protein(AA Leu 25-Gln 167)(Accession # NP_005009.2) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 42.1 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-1, Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-1, Fc Tag, low endotoxin was more than 90% and the molecular weight of this protein is around 130-150 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) at 1 ug/mL (100 uL/well) on streptavidin precoated (0.2 ug/well) plate, can bind Human PD-1, Fc Tag, low endotoxin with a linear range of 0.1-3 ng/mL.

Bioactivity-ELISA 2

Immobilized Human PD-1, Fc Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Biotinylated Human PD-L2, Fc,Avitag with a linear range of 0.5-16 ng/mL.

Bioactivity-ELISA 3

Immobilized Biotinylated Human PD-L2, Avitag,His Tag (recommended for biopanning) at 1 ug/mL (100 uL/well) on streptavidin precoated (0.2 ug/well) plate, can bind Human PD-1, Fc Tag, low endotoxin with a linear range of 0.1-1 ng/mL.

Bioactivity-ELISA 4

Serial dilutions of nivolumab were added into Human PD-1, Fc Tag, low endotoxin: Biotinylated Human PD-L1, Fc,Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 ug/mL.

Bioactivity-BLI 1

Loaded Human PD-1, Fc Tag, low endotoxin on ProteinA Biosensor, can bind Human PD-L1, His Tag (HPLC & BLI verified) with an affinity constant of 5.3 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-1, Fc Tag, low endotoxin on Protein A Biosensor, can bind Human PD-L2, His Tag (SPR verified) with an affinity constant of 0.45 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that Human PD-1, Fc Tag, low endotoxin can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 ug/mL.

Bioactivity-SPR 1

Human PD-1, Fc Tag, low endotoxin (HPLC-verified) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (HPLC-verified) with an affinity constant of 3.6 uM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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