Recombinant Human PDCD1 protein, C-hFc-Avi-His Tag, Biotinylated

Product Description

Cat

IMP-054

Official Symbol

PDCD1

Product Overview

Biotinylated Recombinant Human PDCD1 protein(AA Leu 25-Gln 167)(Accession # Q15116-1) is expressed from human HEK293 cells. This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag is inserted in-between the Fc and his tags.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 44.7 kDa. The protein migrates as 50-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100-130 kDa under non-reducing (NR) condition.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Biotinylated Human PD-1, Fc,Avitag,His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human PD-L1, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag with a linear range of 16-125 ng/mL.

Bioactivity-ELISA 2

Immobilized Nivolumab at 2 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag with a linear range of 0.4-6 ng/mL.

Bioactivity-ELISA 3

Serial dilutions of Anti-PD-L1 Neutralizing Antibody were added into Human PD-L1, Fc Tag (HPLC-verified): Biotinylated Human PD-1, Fc,Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2966 ug/mL.

Bioactivity-BLI 1

Loaded Biotinylated Human PD-1, Fc,Avitag,His Tag on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) with an affinity constant of 2.2 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Biotinylated Human PD-1, Fc,Avitag,His Tag on SA Biosensor, can bind Human PD-L2, His Tag (SPR verified) with an affinity constant of 0.36 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that recombinant Biotinylated Human PD-1, Fc,Avitag,His Tag can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Biotinylated Human PD-1, Fc,Avitag,His Tag to 293 overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-PD-L1 antibody. The concentration of PD-1 used is 1 ug/mL. The IC50 is 0.362 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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