Recombinant Human PDCD1 protein, C-hFc-Avi-His Tag, Biotinylated

Product Description

Cat

IMP-054

Official Symbol

PDCD1

Product Overview

Biotinylated Recombinant Human PDCD1 protein(AA Leu 25-Gln 167)(Accession # Q15116-1) is expressed from human HEK293 cells. This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag is inserted in-between the Fc and his tags.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 44.7 kDa. The protein migrates as 50-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100-130 kDa under non-reducing (NR) condition.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Biotinylated Human PD-1, Fc,Avitag,His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human PD-L1, Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag with a linear range of 16-125 ng/mL.

Bioactivity-ELISA 2

Immobilized Nivolumab at 2 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, Fc,Avitag,His Tag with a linear range of 0.4-6 ng/mL.

Bioactivity-ELISA 3

Serial dilutions of Anti-PD-L1 Neutralizing Antibody were added into Human PD-L1, Fc Tag (HPLC-verified): Biotinylated Human PD-1, Fc,Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2966 ug/mL.

Bioactivity-BLI 1

Loaded Biotinylated Human PD-1, Fc,Avitag,His Tag on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) with an affinity constant of 2.2 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Biotinylated Human PD-1, Fc,Avitag,His Tag on SA Biosensor, can bind Human PD-L2, His Tag (SPR verified) with an affinity constant of 0.36 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that recombinant Biotinylated Human PD-1, Fc,Avitag,His Tag can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Biotinylated Human PD-1, Fc,Avitag,His Tag to 293 overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-PD-L1 antibody. The concentration of PD-1 used is 1 ug/mL. The IC50 is 0.362 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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