Recombinant Human PDCD1 protein, C-His Tag

Product Description

Cat

IMP-629

Official Symbol

PDCD1

Product Overview

Recombinant Human PDCD1 protein(NP_005009.2)(Leu 25-Leu 288), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 30.7 kDa. The protein migrates as 40-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Leu 288

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>80% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human PD-1 Full Length, His Tag at 0.2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.4-3 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of nivolumab were added into Human PD-1 Full Length, His Tag : Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin binding reactions. The half maximal inhibitory concentration (IC50) is 0.14891.

Bioactivity-ELISA 3

Immobilized Human PD-1 Full Length, His Tag at 0.2 μg/mL (100 μL/well) can bind Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin with a linear range of 0.01-0.313 μg/mL.

Bioactivity-ELISA 4

Immobilized Human PD-1 Full Length, His Tag at 0.2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 0.5-8 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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