Recombinant Human PDCD1 protein, C-His Tag

Product Description

Cat

IMP-629

Official Symbol

PDCD1

Product Overview

Recombinant Human PDCD1 protein(NP_005009.2)(Leu 25-Leu 288), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 30.7 kDa. The protein migrates as 40-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Leu 288

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>80% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human PD-1 Full Length, His Tag at 0.2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.4-3 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of nivolumab were added into Human PD-1 Full Length, His Tag : Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin binding reactions. The half maximal inhibitory concentration (IC50) is 0.14891.

Bioactivity-ELISA 3

Immobilized Human PD-1 Full Length, His Tag at 0.2 μg/mL (100 μL/well) can bind Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin with a linear range of 0.01-0.313 μg/mL.

Bioactivity-ELISA 4

Immobilized Human PD-1 Full Length, His Tag at 0.2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 0.5-8 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry