Recombinant Human PDCD1 protein, C-His Tag

Product Description

Cat

IMP-053

Official Symbol

PDCD1

Product Overview

Recombinant Human PDCD1 protein(AA Leu 25-Gln 167)(Accession # NP_005009.2) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 31-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-1, His Tag was more than 85% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human PD-1, His Tag at 2 ug/mL (100 uL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL.

Bioactivity-ELISA 2

Immobilized Human PD-1, His Tag at 2 ug/mL (100 uL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.

Bioactivity-BLI 1

Loaded Human PD-1, His Tag on HIS1K Biosensor, can bind Human PD-L1, Fc Tag (HPLC verified) with an affinity constant of 38.9 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-1, His Tag on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human PD-1, His Tag on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 4

Loaded Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) on AMC Biosensor, can bind Human Human PD-1, His Tag with an affinity constant of 2.1 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Opdivo (Nivolumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-1, His Tag with an affinity constant of 4.94 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry