Recombinant Human PDCD1 protein, C-His Tag

Product Description

Cat

IMP-053

Official Symbol

PDCD1

Product Overview

Recombinant Human PDCD1 protein(AA Leu 25-Gln 167)(Accession # NP_005009.2) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 31-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-1, His Tag was more than 85% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human PD-1, His Tag at 2 ug/mL (100 uL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL.

Bioactivity-ELISA 2

Immobilized Human PD-1, His Tag at 2 ug/mL (100 uL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.

Bioactivity-BLI 1

Loaded Human PD-1, His Tag on HIS1K Biosensor, can bind Human PD-L1, Fc Tag (HPLC verified) with an affinity constant of 38.9 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-1, His Tag on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human PD-1, His Tag on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 4

Loaded Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) on AMC Biosensor, can bind Human Human PD-1, His Tag with an affinity constant of 2.1 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Opdivo (Nivolumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-1, His Tag with an affinity constant of 4.94 nM as determined in a SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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