Recombinant Human PDCD1 protein, C-His Tag

Product Description

Cat

IMP-145

Official Symbol

PDCD1

Product Overview

Recombinant Human PDCD1 protein(AA Leu 25-Gln 167)(Accession # NP_005009.2) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 16.8 kDa. The N-terminus Sequence Analysis is Leu 25. The reducing (R) protein migrates as 31-44 kDa in SDS-PAGE due to glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 0.01 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-1, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-1, His Tag, low endotoxin was more than 85% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human PD-1, His Tag, low endotoxin at 2 ug/mL (100 uL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL.

Bioactivity-BLI 1

Loaded Human PD-1, His Tag, low endotoxin on HIS1K Biosensor, can bind Human PD-L1, Fc Tag with an affinity constant of 23.3 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-1, His Tag, low endotoxin on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC-verified) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human PD-L1, Fc Tag on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin with an affinity constant of 4.8 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 4

Loaded Human PD-L2, Fc Tag (HPLC verified) on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin with an affinity constant of 0.9 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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