Recombinant Human PDCD1 protein, C-mFc Tag

Product Description

Cat

IMP-120

Official Symbol

PDCD1

Product Overview

Recombinant Human PDCD1 protein(AA Leu 25-Gln 167)(Accession # Q15116-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 25-Gln 167

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA 1

Immobilized Human PD-L1, Fc Tag (HPLC-verified) at 10 ug/mL (100 uL/well) can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) with a linear range of 2-40 ng/mL.

Bioactivity-ELISA 2

Immobilized Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Human PD-L2, Fc Tag with a linear range of 0.2-4 ng/mL.

Bioactivity-ELISA 3

Immobilized Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) at 2 ug/mL (100 uL/well) can bind Nivolumab with a linear range of 0.1-2 ng/mL.

Bioactivity-ELISA 4

Immobilized Human PD-L1, His Tag (HPLC-verified) at series of concentration on MonRabTM Anti-His Tag (C-term) Antibody precoated (0.1 ug/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) at 5 ug/mL (100 uL/well) with a linear range of 10-78 ng/mL.

Bioactivity-BLI 1

Loaded Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human PD-L1, His Tag (HPLC verified) with an affinity constant of 2.6 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human PD-L2, His Tag (SPR verified) with an affinity constant of 0.43 uM as determined in BLI assay (ForteBio Octet Red96e).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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