Human PD-1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human PD-1, Strep Tag at 2 ug/mL (100 uL/well) can bind Human PD-L1, Fc Tag with a linear range of 4-63 ng/mL.
Immobilized Human PD-1, Strep Tag at 2 ug/mL (100 uL/well) can bind Human PD-L2, Fc Tag with a linear range of 0.4-6 ng/mL.
Immobilized Human PD-1, Strep Tag at 2 ug/mL (100 uL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.
Loaded Human PD-L1, Fc Tag (HPLC-verified) on Protein A Biosensor, can bind Human Human PD-1, Strep Tag with an affinity constant of 2.5 uM as determined in BLI assay (ForteBio Octet Red96e).
Loaded Human PD-L2, Fc Tag (HPLC verified) on Protein A Biosensor, can bind Human PD-1, Strep Tag with an affinity constant of 0.42 uM as determined in BLI assay (ForteBio Octet Red96e).