Recombinant Human PDCD1LG2 protein, C-Fc Tag

Product Description

Cat

IMP-046

Official Symbol

PDCD1LG2

Product Overview

Recombinant Human PDCD1LG2 protein(AA Leu 20-Pro 219)(Accession # AAI13679) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 20-Pro 219

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-L2, Fc Tag (HPLC verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HPLC

The purity of Human PD-L2, Fc Tag (HPLC verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-BLI 1

Loaded Human PD-1, His Tag (HPLC verified) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-1, His Tag, low endotoxin (HPLC-verified) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC-verified) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human PD-L2, Fc Tag (HPLC verified) on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin (HPLC-verified) with an affinity constant of 0.9 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 4

Loaded Human PD-L2, Fc Tag (HPLC verified) on Protein A Biosensor, can bind Human PD-1, Strep Tag with an affinity constant of 0.42 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that recombinant Human PD-L2, Fc Tag (HPLC verified) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 ug/mL.

Bioactivity-Cell based assay 1

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2, Fc Tag (HPLC verified) with an EC50 of 0.018 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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