Recombinant Human PDCD1LG2 protein, C-Fc Tag

Product Description

Cat

IMP-046

Official Symbol

PDCD1LG2

Product Overview

Recombinant Human PDCD1LG2 protein(AA Leu 20-Pro 219)(Accession # AAI13679) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 20-Pro 219

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human PD-L2, Fc Tag (HPLC verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HPLC

The purity of Human PD-L2, Fc Tag (HPLC verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-BLI 1

Loaded Human PD-1, His Tag (HPLC verified) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human PD-1, His Tag, low endotoxin (HPLC-verified) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC-verified) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 3

Loaded Human PD-L2, Fc Tag (HPLC verified) on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin (HPLC-verified) with an affinity constant of 0.9 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 4

Loaded Human PD-L2, Fc Tag (HPLC verified) on Protein A Biosensor, can bind Human PD-1, Strep Tag with an affinity constant of 0.42 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

Flow Cytometry assay shows that recombinant Human PD-L2, Fc Tag (HPLC verified) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 ug/mL.

Bioactivity-Cell based assay 1

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2, Fc Tag (HPLC verified) with an EC50 of 0.018 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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