Recombinant Human PDCD1LG2 protein, C-mFc Tag

Product Description

Cat

IMP-045

Official Symbol

PDCD1LG2

Product Overview

Recombinant Human PDCD1LG2 protein(AA Leu 20-Pro 219)(Accession # AAI13679) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 48.9 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 20-Pro 219

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human PD-L2, Mouse IgG1 Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human PD-1, His Tag at 2 ug/mL (100 uL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of nivolumab were added into Human PD-L2, Mouse IgG1 Fc Tag: Biotinylated Human PD-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2823 ug/mL.

Bioactivity-FACS 1

Flow Cytometry assay shows that Human PD-L2, Mouse IgG1 Fc Tag can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 0.1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human PD-L2, Mouse IgG1 Fc Tag to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing Anti-PD-1 antibody. The concentration of PD-L2 used is 0.1 ug/mL. The IC50 is 1.3 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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