Recombinant Human PDGFRB Protein, C-His-tagged, Biotinylated

Product Description

Cat

IMP-4891

Official Symbol

PDGFRB

Product Overview

Recombinant human PDGFRβ (NP_002600.1) extracellular domain (Met1-Lys531) was expressed, with a C-terminal polyhistidine tag. The expressed protein was biotinylated in vitro.

Description

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.

Expression System

HEK293

Species

Human

Tag

C-His

Predicted N Terminal

Leu 33

Form

Lyophilized from sterile Sterile PBS, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human PDGFRβ comprises 510 amino acids and predicts a molecular mass of 57.6 kDa.

Protein length

Met1-Lys531

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized biotinylated human PDGFRb-His at 10μg/mL (100 μL/well) can bind Cynomolgus PDGFB-Fc, The EC50 of Cynomolgus PDGFB-Fc is 30-80ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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