Recombinant Human PECAM1 Protein (Gln28-Lys601), Tag Free
Product Description
Cat
IMP-10008
Official Symbol
PECAM1
Product Overview
Recombinant human CD31/PECAM-1 protein (Gln28-Lys601) without tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Expression System
CHO
Species
Human
Tag
Tag Free
Predicted N Terminal
No results obtained: Gln28 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Molecular Mass
Predicted Molecular Mass: 64.5 kDa
Protein length
Gln28-Lys601
Bio-activity
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5 × 10^4 cells/well are added to rhCD31 coated plates (3.3 μg/mL, 100 μL/well) in the presence of 20 ng.mL PMA, approximately 50-65% will adhere after 30 minutes at 37 centigrade.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 150 μg/mL in sterile, deionized water.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info