Recombinant Human PECAM1 Protein (Gln28-Lys601), Tag Free
Product Description
Cat
IMP-10008
Official Symbol
PECAM1
Product Overview
Recombinant human CD31/PECAM-1 protein (Gln28-Lys601) without tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Expression System
CHO
Species
Human
Tag
Tag Free
Predicted N Terminal
No results obtained: Gln28 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Molecular Mass
Predicted Molecular Mass: 64.5 kDa
Protein length
Gln28-Lys601
Bio-activity
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5 × 10^4 cells/well are added to rhCD31 coated plates (3.3 μg/mL, 100 μL/well) in the presence of 20 ng.mL PMA, approximately 50-65% will adhere after 30 minutes at 37 centigrade.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 150 μg/mL in sterile, deionized water.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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