Recombinant Human PVR Protein (Asp28-Asn343), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10082
Official Symbol
PVR
Product Overview
Biotinylated recombinant human CD155/PVR protein (Asp28-Asn343) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Asp28
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 63 kDa
SDS-PAGE: 86-98 kDa, reducing conditions
Protein length
Asp28-Asn343
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD96v2 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag binds with an ED50 of 10-60 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD96v2 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag binds with an ED50 of 10-60 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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