Recombinant Human PVR Protein (Asp28-Asn343), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10082
Official Symbol
PVR
Product Overview
Biotinylated recombinant human CD155/PVR protein (Asp28-Asn343) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.
Description
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
Expression System
HEK293
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Asp28
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 63 kDa
SDS-PAGE: 86-98 kDa, reducing conditions
Protein length
Asp28-Asn343
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD96v2 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag binds with an ED50 of 10-60 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD96v2 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag binds with an ED50 of 10-60 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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