Recombinant Human PVR protein, C-hFc-Avi Tag, Biotinylated

Product Description

Cat

IMP-079

Official Symbol

PVR

Product Overview

Biotinylated Recombinant Human PVR protein(AA Trp 21-Asn 343)(Accession # NP_006496.2) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 63.5 kDa. As a result of glycosylation, the protein migrates as 75-100 kDa under reducing (R) condition, and 140-180 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Trp 21-Asn 343

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Biotinylated Human CD155, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human TIGIT, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD155, Fc,Avitag with a linear range of 0.5-16 ng/mL.

Bioactivity-ELISA 2

Immobilized Human DNAM-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD155, Fc,Avitag with a linear range of 0.02-0.312 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 ug/mL to 0.0097 ug/mL) were added into Human TIGIT, His Tag: Biotinylated Human CD155, Fc,Avitag binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

Bioactivity-FACS 1

FACS assay shows that Biotinylated Human CD155, Fc,Avitag can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Biotinylated Human CD155, Fc,Avitag to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 ug/mL. The IC50 is 0.201 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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