Recombinant Human PVR protein, C-hFc-Avi Tag, Biotinylated

Product Description

Cat

IMP-079

Official Symbol

PVR

Product Overview

Biotinylated Recombinant Human PVR protein(AA Trp 21-Asn 343)(Accession # NP_006496.2) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Expression System

HEK293

Species

Human

Tag

C-hFc-Avi Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Conjugate

Biotinylated

Molecular Mass

The protein has a calculated MW of 63.5 kDa. As a result of glycosylation, the protein migrates as 75-100 kDa under reducing (R) condition, and 140-180 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Trp 21-Asn 343

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Biotinylated Human CD155, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human TIGIT, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD155, Fc,Avitag with a linear range of 0.5-16 ng/mL.

Bioactivity-ELISA 2

Immobilized Human DNAM-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD155, Fc,Avitag with a linear range of 0.02-0.312 ug/mL.

Bioactivity-ELISA 3

Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 ug/mL to 0.0097 ug/mL) were added into Human TIGIT, His Tag: Biotinylated Human CD155, Fc,Avitag binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

Bioactivity-FACS 1

FACS assay shows that Biotinylated Human CD155, Fc,Avitag can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Biotinylated Human CD155, Fc,Avitag to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 ug/mL. The IC50 is 0.201 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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