Recombinant Human PVR protein, C-mFc Tag

Product Description

Cat

IMP-080

Official Symbol

PVR

Product Overview

Recombinant Human PVR protein(AA Trp 21-Asn 343)(Accession # NP_006496.4) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

The protein has a calculated MW of 62.2 kDa. As a result of glycosylation, the protein migrates as 70-100 kDa under reducing (R) condition, and 130-160 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Trp 21-Asn 343

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human CD155, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human TIGIT, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.1-4 ng/mL.

Bioactivity-ELISA 2

Immobilized Human DNAM-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 1-16 ng/mL.

Bioactivity-ELISA 3

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Mouse IgG2a Fc Tag, low endotoxin: Biotinylated Human TIGIT, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.08116 ug/mL.

Bioactivity-BLI 1

Loaded Human CD155, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.24 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human CD155, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human DNAM-1, His Tag with an affinity constant of 0.57 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS assay shows that Human CD155, Mouse IgG2a Fc Tag, low endotoxin can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human CD155, Mouse IgG2a Fc Tag, low endotoxin to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 ug/mL. The IC50 is 0.1413 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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