Recombinant Human PVR protein, C-mFc Tag

Product Description

Cat

IMP-080

Official Symbol

PVR

Product Overview

Recombinant Human PVR protein(AA Trp 21-Asn 343)(Accession # NP_006496.4) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

The protein has a calculated MW of 62.2 kDa. As a result of glycosylation, the protein migrates as 70-100 kDa under reducing (R) condition, and 130-160 kDa under non-reducing (NR) condition (SDS-PAGE).

Protein length

Trp 21-Asn 343

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human CD155, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human TIGIT, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.1-4 ng/mL.

Bioactivity-ELISA 2

Immobilized Human DNAM-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 1-16 ng/mL.

Bioactivity-ELISA 3

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Mouse IgG2a Fc Tag, low endotoxin: Biotinylated Human TIGIT, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.08116 ug/mL.

Bioactivity-BLI 1

Loaded Human CD155, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.24 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Loaded Human CD155, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human DNAM-1, His Tag with an affinity constant of 0.57 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS assay shows that Human CD155, Mouse IgG2a Fc Tag, low endotoxin can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human CD155, Mouse IgG2a Fc Tag, low endotoxin to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 ug/mL. The IC50 is 0.1413 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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