Recombinant Human PVRIG Protein (Thr41 & Glu43-Leu172), C-hFc-tagged

Product Description

Cat

IMP-9959

Official Symbol

PVRIG

Product Overview

Recombinant human PVRIG protein (Thr41 & Glu43-Leu172) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Description

Enables phosphatase binding activity and signaling receptor activity. Involved in negative regulation of T cell receptor signaling pathway. Located in plasma membrane.

Expression System

HEK293

Species

Human

Tag

C-hFc

Predicted N Terminal

Thr41&Glu43

Form

Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and PEG with Trehalose.

Molecular Mass

Predicted Molecular Mass: 40 kDa
SDS-PAGE: 51-57 kDa, reducing conditions

Protein length

Thr41 & Glu43-Leu172

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human PVRIG Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human Nectin-2/CD112 that produces 50% of the optimal binding response is 0.4-2.4 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in sterile water.

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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