Recombinant Human RAET1E Protein (Gly30-Asp225), C-hFc-tagged
Product Description
Cat
IMP-9857
Official Symbol
RAET1E
Product Overview
Recombinant human ULBP-4/RAET1E protein (Gly30-Asp225) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene belong to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3. This and RAET1G protein differ from other RAET1 proteins in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. This protein functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc
Predicted N Terminal
Gly30
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 49 kDa (monomer)
SDS-PAGE: 60-65 kDa, reducing conditions
Protein length
Gly30-Asp225
Bio-activity
Measured by the ability of the immobilized protein to induce IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is typically 2-10 μg/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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