Recombinant Human RAET1L Protein (26-218aa), C-His-Tagged
Product Description
Cat
IMP-9531
Official Symbol
RAET1L
Product Overview
Recombinant human RAET1L, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description
RAET1L, also known as retinoic acid early transcript 1L, linked to the cell membrane by the GPI anchor. This protein functions as a stress-induced ligand for NKG2D receptor. Its expression is, for example, triggered in course of HCMV infection, but HCMV alters its function. HCMV-encoded UL16 glycoprotein retains RAET1L/ULBP6 inside the cells, preventing it from reaching the cell surface and being exposed to cells of the immune system. It had a more restricted expression profile in cell lines and primary human tissues than other NKG2D ligands. RAET1L mRNA shows restricted expression in the trachea and some HPV positive cervical carcinoma and colorectal carcinoma cell lines.
Expression System
Baculovirus
Species
Human
Tag
C-His
Form
Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Molecular Mass
22.9 kDa (202 aa)
Protein length
26-218
AA Sequence
<ADP>RRDDPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGNKTVTPVSPLGKKLNVTMAWKAQNPVLREVVDILTEQLLDIQLENYTPKEPLTLQARMSCEQKAEGHSSGSWQFSIDGQTFLLFDSEKRMWTTVHPGARKMKEKWENDKDVAMSFHYISMGDCIGWLEDFLMGMDSTLEPSAGAPLAMSSG<HHHHHH>
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Applications
SDS-PAGE, Bioactivity
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/mL (determined by Absorbance at 280nm)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1
Biological Activity

Human RAET1L/ULBP6 is coated at 10 μg/mL (100 μL/well) can bind Human NKG2D in a Functional ELISA assay

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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