IMP-4454

ERBB4

Recombinant human / rhesus ERBB4 (NP_005226.1) (Met1-Arg649) with a C-terminal polyhistidine tag was expressed. Human and Rhesus ERBB4 sequences are identical.

ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.

HEK293

Human, Rhesus

C-His

Gln 26

Lyophilized from sterile PBS, pH7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human / rhesus ERBB4 comprises 635 amino acids and has a predicted molecular mass of 71.1 kDa. The apparent molecular mass of the protein is approximately 102 kDa in SDS-PAGE under reducing conditions.

Met1-Arg649

< 1.0 EU/μg of the protein as determined by the LAL method

> 85 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Measured by its binding ability in a functional ELISA. Immobilized human ErbB4-His at 10 μg/mL (100 μL/well) can bind biotinylated human NRG1, The EC50 of biotinylated human NRG1 is 0.4-0.92 μg/mL.