Recombinant Human RNF43 Protein, C-hFc-tagged

Product Description

Cat

IMP-1767

Official Symbol

RNF43

Product Overview

Recombinant human RNF43 (NP_060233.3) (Met1-Tyr197) was expressed with the Fc region of human IgG1 at the C-terminus.

Description

RNF43 mutations are frequently detected in colorectal cancer cells and lead to a loss of function of the ubiquitin E3 ligase. The outer mitochondrial membrane 34 (TOMM34) and ring finger protein 43 (RNF43) as highly expressed oncogenes in malignant colorectal tumors. RNF43 is a tumour suppressor gene that suppresses the Wnt-beta-catenin signalling pathway.

Expression System

HEK293

Species

Human

Tag

C-hFc

Predicted N Terminal

Gly 24

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human RNF43/Fc comprises 412 amino acids and has a predicted molecular mass of 45.8 kDa. The apparent molecular mass of the protein is approximately 52 kDa in SDS-PAGE under reducing conditions.

Protein length

Met1-Tyr197

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized Human RSPO3 His at 2 μg/mL (100 μL/well) can bind Human RNF43 hFc, the EC50 of Human RNF43 hFc is 3.0-12.0 ng/mL

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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