Recombinant Human RPS27A Protein (1-76aa), Tag Free

Product Description

Cat

IMP-9548

Official Symbol

RPS27A

Product Overview

ubiquitin (76 amino acid) was overexpressed in E. coli and purified by using conventional chromatography techniques.

Description

ubiquitin is a small protein that is composed of 76 amino acids and its sequence is highly conserved throughout evolution from invertebrates to mammals. In the cytoplasm, ubiquitin is involved in ATP-dependent nonlysomal proteolysis of various proteins. In the nucleus, ubiquitin is conjugated to histone2A and may play a role in regulation of chromatin structure and/or regulation of transcriptional activity.

Expression System

E. coli

Species

Human

Tag

Tag Free

Form

Liquid in 50mM HEPES buffer (pH 7.4) containing 150mM NaCl, 1mM DTT, 10% glycerol

Molecular Mass

8.6 kDa (76 aa) confirmed by MALDI-TOF

Protein length

1-76

AA Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Applications

SDS-PAGE

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

1 mg/mL (determined by BCA assay)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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