Recombinant Human RPS27A Protein (1-76aa), Tag Free

Product Description

Cat

IMP-9548

Official Symbol

RPS27A

Product Overview

ubiquitin (76 amino acid) was overexpressed in E. coli and purified by using conventional chromatography techniques.

Description

ubiquitin is a small protein that is composed of 76 amino acids and its sequence is highly conserved throughout evolution from invertebrates to mammals. In the cytoplasm, ubiquitin is involved in ATP-dependent nonlysomal proteolysis of various proteins. In the nucleus, ubiquitin is conjugated to histone2A and may play a role in regulation of chromatin structure and/or regulation of transcriptional activity.

Expression System

E. coli

Species

Human

Tag

Tag Free

Form

Liquid in 50mM HEPES buffer (pH 7.4) containing 150mM NaCl, 1mM DTT, 10% glycerol

Molecular Mass

8.6 kDa (76 aa) confirmed by MALDI-TOF

Protein length

1-76

AA Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

Endotoxin

< 1 EU/μg of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Applications

SDS-PAGE

Notes

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

Storage

Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.

Concentration

1 mg/mL (determined by BCA assay)

SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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