Recombinant Human RSPO1 Protein, C-His-tagged

Product Description

Cat

IMP-1385

Official Symbol

RSPO1

Product Overview

Recombinant human RSPO1 (NP_001033722.1) (Met 1-Ala 263) was expressed fused with a polyhistidine tag at the C-terminus.

Description

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

Expression System

HEK293

Species

Human

Tag

C-His

Predicted N Terminal

Ser 21

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The secreted recombinant human RSPO1 comprises 254 amino acids with a predicted molecular mass of 28.2 kDa. As a result of glycosylation, rhRSPO1 migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.

Protein length

Met1-Ala263

Endotoxin

< 1.0 EU/μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

1. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/mL (100 μL/well) can bind human LIMPII with a linear range of 32-800 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/mL (100 μL/well) can bind mouse CD36 with a linear range of 6.4-800 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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