Recombinant Human RSPO2 Protein, C-hFc-tagged
Product Description
Cat
IMP-1520
Official Symbol
RSPO2
Product Overview
Recombinant Human RSPO2 (Q6UXX9-1, with mutations L186P) (Met1-Gly205) was expressed with the Fc region of human IgG1 at the C-terminus.
Description
R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt / beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled / LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2 / Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2 / Wnt1 tumor cells were transferred into naive mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors.
Expression System
HEK293
Species
Human
Tag
C-hFc
Predicted N Terminal
Gln 22
Form
Lyophilized from sterile Histidine, Arginine, 120mM NaCl, 0.02% Tween 80, 5% sucrose, pH 6.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human RSPO2 consists of 422 amino acids and predicts a molecular mass of 48.01 kDa. It migrates as an approximately 56.15 kDa band in SDS-PAGE under reducing conditions.
Protein length
Met1-Gly205
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 2-12 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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