Recombinant Human RSPO3 Protein (Gln22-His272)
Product Description
Cat
IMP-9769
Official Symbol
RSPO3
Product Overview
Recombinant human R-Spondin 3 protein (Gln22-His272) without tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
Expression System
CHO
Species
Human
Tag
Tag Free
Predicted N Terminal
No results obtained: Gln22 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 28.3 kDa
SDS-PAGE: 36-45 kDa, reducing conditions
Protein length
Gln22-His272
Bio-activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.5-2.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS containing at least 0.1% of human or bovine serun albumin.
Bioactivity-ELISA 1
Binding Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.5-2.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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