Recombinant Human RSPO3 Protein (Gln22-His272)
Product Description
Cat
IMP-9769
Official Symbol
RSPO3
Product Overview
Recombinant human R-Spondin 3 protein (Gln22-His272) without tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
Expression System
CHO
Species
Human
Tag
Tag Free
Predicted N Terminal
No results obtained: Gln22 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 28.3 kDa
SDS-PAGE: 36-45 kDa, reducing conditions
Protein length
Gln22-His272
Bio-activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.5-2.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS containing at least 0.1% of human or bovine serun albumin.
Bioactivity-ELISA 1
Binding Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.5-2.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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