Description
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
Bio-activity
Measured by its binding ability in a functional ELISA. When Human Syndecan-1/CD138 Antibody is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human Syndecan-1/CD138 Fc Chimera Avi-tag binds with an ED50 of 0.3-2.1 ng/mL. Measured by the ability of the immobilized protein to support the adhesion of Saos-2 human osteosarcoma cells. The ED50 for this effect is 0.5-6.0 μg/mL.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
