Recombinant Human SIGLEC10 Protein (Met17-Thr546), C-hFc and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10068
Official Symbol
SIGLEC10
Product Overview
Biotinylated recombinant human Siglec-10 protein (Met17-Thr546) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6.
Expression System
CHO
Species
Human
Tag
C-hFc and Avi
Predicted N Terminal
Met17
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 87 kDa
SDS-PAGE: 95-118 kDa, under reducing conditions
Protein length
Met17-Thr546
Bio-activity
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD52 Fc Chimera Protein is immobilized at 5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Siglec-10 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 0.75-4.50 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD52 Fc Chimera Protein is immobilized at 5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Siglec-10 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 0.75-4.50 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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