Recombinant Human SIGLEC10 Protein (Met17-Thr546), C-hFc and Avi-tagged, Biotinylated

Product Description

Cat

IMP-10068

Official Symbol

SIGLEC10

Product Overview

Biotinylated recombinant human Siglec-10 protein (Met17-Thr546) with a Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Description

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6.

Expression System

CHO

Species

Human

Tag

C-hFc and Avi

Predicted N Terminal

Met17

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 87 kDa
SDS-PAGE: 95-118 kDa, under reducing conditions

Protein length

Met17-Thr546

Bio-activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD52 Fc Chimera Protein is immobilized at 5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Siglec-10 Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 0.75-4.50 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer, biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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