Recombinant Human SIGLEC10 Protein (Met17-Thr546), C-hFc-tagged

Product Description

Cat

IMP-9715

Official Symbol

SIGLEC10

Product Overview

Recombinant human Siglec-10 protein (Met17-Thr546) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-hFc

Predicted N Terminal

Met17

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 85 kDa (monomer)
SDS-PAGE: 105-110 kDa, reducing conditions

Protein length

Met17-Thr546

Bio-activity

Measured by its ability to bind biotinylated 6'-Sialyllactose-Polyacrylamide in functional ELISA. Whitney, G. et al. (2001) FEBS 268:6083. rhSiglec-10/Fc Chimera immobilized at 5 μg/mL (100 μL/well) on a goat anti-human IgG Fc antibody coated plate can bind biotinylated 6'-Sialyllactose-Polyacrylamide with a linear range of 0.04-2.5 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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