Recombinant Human SIGLEC10 Protein (Met17-Thr546), C-hFc-tagged

Product Description

Cat

IMP-9715

Official Symbol

SIGLEC10

Product Overview

Recombinant human Siglec-10 protein (Met17-Thr546) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-hFc

Predicted N Terminal

Met17

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 85 kDa (monomer)
SDS-PAGE: 105-110 kDa, reducing conditions

Protein length

Met17-Thr546

Bio-activity

Measured by its ability to bind biotinylated 6'-Sialyllactose-Polyacrylamide in functional ELISA. Whitney, G. et al. (2001) FEBS 268:6083. rhSiglec-10/Fc Chimera immobilized at 5 μg/mL (100 μL/well) on a goat anti-human IgG Fc antibody coated plate can bind biotinylated 6'-Sialyllactose-Polyacrylamide with a linear range of 0.04-2.5 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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