Recombinant Human SIGLEC15 Protein (Phe20-Thr263), C-hFc-tagged
Product Description
Cat
IMP-9953
Official Symbol
SIGLEC15
Product Overview
Recombinant human Siglec-15 protein (Phe20-Thr263) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
Predicted to be involved in regulation of actin cytoskeleton organization; regulation of bone resorption; and regulation of osteoclast development. Predicted to be integral component of membrane. Predicted to be part of protein-containing complex. Predicted to be active in plasma membrane.
Expression System
CHO
Species
Human
Tag
C-hFc
Predicted N Terminal
Phe20
Form
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in water.
Bioactivity-ELISA 1
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
