Recombinant Human SIGLEC15 Protein (Phe20-Tyr251), C-6×His-tagged
Product Description
Cat
IMP-9621
Official Symbol
SIGLEC15
Product Overview
Recombinant human Siglec-15 protein (Phe20-Tyr251) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
Predicted to be involved in regulation of actin cytoskeleton organization; regulation of bone resorption; and regulation of osteoclast development. Predicted to be integral component of membrane. Predicted to be part of protein-containing complex. Predicted to be active in plasma membrane.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
Phe20
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.2-2 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Bioactivity-ELISA 1
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.2-2 μg/mL.
