Recombinant Human SIGLEC5 Protein (Glu17-Thr434), C-hFc-tagged
Product Description
Cat
IMP-9623
Official Symbol
SIGLEC5
Product Overview
Recombinant human Siglec-5 protein (Glu17-Thr434) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc
Predicted N Terminal
Glu17
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 73 kDa (monomer)
SDS-PAGE: 100-110 kDa, reducing conditions
Protein length
Glu17-Thr434
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 1-4 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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