Description
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs, are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM, and a motif homologous to one identified in signaling lymphocyte activation molecule that mediates an association with SLAM-associated protein.
Bio-activity
Measured by its binding ability in a functional ELISA. When Biotinylated Sialyl Lewis X was immobilized at 0.05 μg/mL (100 μL/well), it binds Recombinant Human Siglec-8 Fc Chimera. The concentration of Human Siglec-8 Fc Chimera that produces 50% of the optimal binding response is approximately 0.2-1.2 μg/mL.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
