Biotinylated Human SIRP alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human SIRP alpha, Fc,Avitag was more than 90% and the molecular weight of this protein is around 155-170 kDa verified by SEC-MALS.
Immobilized Human CD47, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human SIRP alpha, Fc,Avitag with a linear range of 5-205 ng/mL.
Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag: Biotinylated Human SIRP alpha, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 ug/mL.
FACS assay shows that Biotinylated Human SIRP alpha, Fc,Avitag can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 3 ug/mL.
FACS analysis shows that the binding of Biotinylated Human SIRP alpha, Fc,Avitag to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti- Human CD47 antibody. The concentration of SIRP alpha used is 1 ug/mL. IC50=0.1303 ug/mL.