Recombinant Human SIRPA protein, C-hFc-Avi Tag, Biotinylated
Product Description
Cat
IMP-044
Official Symbol
SIRPA
Product Overview
Biotinylated Recombinant Human SIRPA protein(AA Glu 31-Arg 370)(Accession # P78324-1) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.
Expression System
HEK293
Species
Human
Tag
C-hFc-Avi Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Conjugate
Biotinylated
Molecular Mass
The protein has a calculated MW of 65.8 kDa. The protein migrates as 75-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Glu 31-Arg 370
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Bioactivity-FACS Title 2
Bioactivity-FACS
SDS-PAGE
SDS-PAGE

Biotinylated Human SIRP alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1
SEC-MALS

The purity of Biotinylated Human SIRP alpha, Fc,Avitag was more than 90% and the molecular weight of this protein is around 155-170 kDa verified by SEC-MALS.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human CD47, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human SIRP alpha, Fc,Avitag with a linear range of 5-205 ng/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag: Biotinylated Human SIRP alpha, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 ug/mL.

Bioactivity-FACS 1
Bioactivity-FACS

FACS assay shows that Biotinylated Human SIRP alpha, Fc,Avitag can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 3 ug/mL.

Bioactivity-FACS 2
FACS analysis shows that the binding of Biotinylated Human SIRP alpha, Fc,Avitag to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti- Human CD47 antibody. The concentration of SIRP alpha used is 1 ug/mL. IC50=0.1303 ug/mL.

FACS analysis shows that the binding of Biotinylated Human SIRP alpha, Fc,Avitag to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti- Human CD47 antibody. The concentration of SIRP alpha used is 1 ug/mL. IC50=0.1303 ug/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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