Recombinant Human SIRPA protein, C-His-Avi Tag, Biotinylated
Product Description
Cat
IMP-516
Official Symbol
SIRPA
Product Overview
Biotinylated Recombinant Human SIRPA(NP_001035111.1)(Glu31-Arg370) is expressed in HEK293, fused with a 6His, Avi tag at the C-terminus.
Description
Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation.
Expression System
HEK293
Species
Human
Tag
C-His-Avi Tag
Form
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Molecular Mass
50-80kDa, reducing conditions.
Protein length
Glu31-Arg370
Endotoxin
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human SIRPA-His-Avi at 10μg/ml (100 μl/well) can bind Human CD47-Fc. The ED50 of Human CD47-Fc is 898 ng/ml.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
  • 1-631-938-8127
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