Recombinant Human SIRPA protein, C-His Tag

Product Description

Cat

IMP-043

Official Symbol

SIRPA

Product Overview

Recombinant Human SIRPA protein(AA Glu 31-Arg 370)(Accession # NP_001035111) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.5 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Glu 31-Arg 370

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human SIRP alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human SIRP alpha, His Tag was more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag: Biotinylated Human CD47, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.1245 ug/mL.

Bioactivity-BLI 1

Loaded Human CD47, Mouse IgG2a Fc Tag, low endotoxin on Protein A Biosensor, can bind Human SIRP alpha, His Tag with an affinity constant of 1.1 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-BLI 2

Immobilized Human CD47, Fc Tag on AHC Biosensor, can bind Human SIRP alpha, His Tag with an affinity constant of 0.72 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-FACS 1

FACS assay shows that Human SIRP alpha, His Tag can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human SIRP alpha, His Tag to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti-CD47 antibody. The concentration of SIRP alpha used is 1 ug/mL. IC50=0.2257 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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