Recombinant Human SIRPA protein, C-mFc Tag

Product Description

Cat

IMP-041

Official Symbol

SIRPA

Product Overview

Recombinant Human SIRPA protein(AA Glu 31-Arg 370)(Accession # NP_001035111) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-mFc Tag

Form

Lyophilized from sterile 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 63.0 kDa. The protein migrates as 70-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Glu 31-Arg 370

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Bioactivity-FACS Title 2

Bioactivity-FACS

SDS-PAGE

Human SIRP alpha, Mouse IgG1 Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Human SIRP alpha, Mouse IgG1 Fc Tag was more than 90% and the molecular weight of this protein is around 150-165 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Human CD47, His Tag at 2 ug/mL (100 uL/well) can bind Human SIRP alpha, Mouse IgG1 Fc Tag (HPLC-verified) with a linear range of 4-31 ng/mL.

Bioactivity-ELISA 2

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, Mouse IgG1 Fc Tag: Biotinylated Human CD47, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006 ug/mL.

Bioactivity-FACS 1

FACS assay shows that Human SIRP alpha, Mouse IgG1 Fc Tag can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 ug/mL.

Bioactivity-FACS 2

FACS analysis shows that the binding of Human SIRP alpha, Mouse IgG1 Fc Tag to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 ug/mL. IC50=0.1318 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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