Recombinant Human SLAMF7 protein, C-Fc Tag

Product Description

Cat

IMP-019

Official Symbol

SLAMF7

Product Overview

Recombinant Human SLAMF7 protein(AA Ser 23-Met 226)(Accession # AAH27867) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Human

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.0 kDa. The protein migrates as 60-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Ser 23-Met 226

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Human SLAMF7, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Human SLAMF7, Fc Tag at 2 ug/mL (100 uL/well) can bind Humanized Anti-Human CS1 MAb with a linear range of 0.3-5 ng/mL.

Bioactivity-FACS 1

2e5 of SLAMF7-CAR-293 cells transfected with anti-SLAMF7-scFv were stained with 100 uL of 0.3 ug/mL of Human SLAMF7, Fc Tag and negative control protein respectively, washed and then followed by PE anti-human IgG Fc antibody and analyzed with FACS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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