Recombinant Human SLPI Protein, N-His-tagged
Product Description
Cat
IMP-4943
Official Symbol
SLPI
Product Overview
Recombinant secreted form of human SLPI (P03973) (Ser 26-Ala 132) was expressed, with a polyhistidine tag at the N-terminus.
Description
Secretory leukoprotease inhibitor (SLPI), also called antileukoprotease (ALP), is a 12-kDa, nonglycosylated serine protease inhibitor present in mucous secretions. It is thought to play a role in protecting the mucosae from injury associated with inflammation. SLPI is locally produced by serous cells, including bronchial submucosal glands. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites, and have been shown to be modulated in multiple pathological conditions. Elafin and SLPI are structurally related in that both have a fold with a four-disulfide core or whey acidic protein (WAP) domain responsible for inhibiting proteases. SLPI is a prominent innate immune protein of the respiratory tract, possessing serine protease inhibitor activity, antibacterial activity, and anti-inflammatory/immunomodulatory activity.
Expression System
Insect cell
Species
Human
Tag
N-His
Predicted N Terminal
Met
Form
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human SLPI consists of 126 amino acids and predicts a molecular mass of 14 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.
Protein length
Ser26-Ala132
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 84 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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