Recombinant Human SOST Protein, N-His-tagged, Biotinylated

Product Description

Cat

IMP-4946

Official Symbol

SOST

Product Overview

Recombinant SOST (NP_079513.1) (Gln24-Tyr213) was expressed with a polyhistidine tag at the N-terminus. The purified protein was biotinylated in vitro.

Description

Sclerostin, the protein product of the SOST gene, is a potent inhibitor of bone formation. Sclerostin protein is widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days, and was originally identified as an important regulator of bone remodeling, homeostasis, and links bone resorption and bone apposition. Recent studies have revealed that Sclerostin protein inhibits the bone growth probably by binding to the extracellular domain of the Wnt coreceptors LRP5 and LRP6 and disrupting Wnt-induced Frizzled-LRP complex formation.

Expression System

HEK293

Species

Human

Tag

N-His

Predicted N Terminal

His

Form

Lyophilized from sterile Sterile PBS, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant SOST consists of 197 amino acids and predicts a molecular mass of 22.5 kDa.

Protein length

Gln24-Tyr213

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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