Human TGFA recombinant protein with polyhistidine tag at the C-terminus expressed in E. coli.
Description
Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF) and competes with EGF for binding to the EGF receptor, stimulating its phosphorylation and producing a mitogenic response.
Expression System
E. coli
Species
Human
Tag
C-His
Form
Lyophilized from a solution containing 1X PBS, pH 8.0. Reconstitute the lyophilized powder in ddH2O to 100 μg/mL.
AA Sequence
MVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA with polyhistidine tag at the C-terminus.
Endotoxin
< 0.1 EU/μg of protein by the LAL method.
Purity
> 98% by SDS-PAGE
Applications
Functional Study; SDS-PAGE
Storage
Lyophilized protein should be stored at -20 centigrade. Protein aliquots should be stored at-20 to -80 centigrade. This product is stable for one year. Avoid repeated freeze/thaw cycles.
SDS-PAGE
SDS-PAGE Stained with Coomassie Blue. SDS-PAGE analysis of TGFA (Human) Recombinant Protein.
