Recombinant Human TGFB1 Protein, C-His-tagged
Product Description
Cat
IMP-8931
Official Symbol
TGFB1
Product Overview
Human TGFB1 recombinant protein with polyhistidine tag at the C-terminus expressed in E. coli.
Description
TGFB is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. TGFB1, TGFB2, and TGFB3 all function through the same receptor signaling systems.
Expression System
E. coli
Species
Human
Tag
C-His
Form
Lyophilized from PBS, pH 8.0.
AA Sequence
MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
Endotoxin
< 0.1 EU/μg of the protein by the LAL method.
Purity
> 98% by SDS-PAGE
Applications
Functional Study; SDS-PAGE
Storage
Store at -20 centigrade, lyophilized protein is stable for 1 year. After reconstitution with deionized water, store at -20 to -80 centigrade. Aliquot to avoid repeated freezing and thawing.
SDS-PAGE
Quality Control Testing

SDS-PAGE Stained with Coomassie Blue

Bioactivity-ELISA 1
Functional Study

Measure by its ability to induce proliferation in MCF-7 cells The ED50 for this effect is <3.2 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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