Recombinant Human TGFB1 Protein (Leu30-Ser390)
Product Description
Cat
IMP-9747
Official Symbol
TGFB1
Product Overview
Recombinant human Latent TGF-beta 1 protein (Leu30-Ser390) without tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
Expression System
CHO
Species
Human
Tag
Tag Free
Predicted N Terminal
Leu30 (LAP)&Ala279 (Mature)
Form
Supplied as a 0.2 μm filtered solution in PBS and Glycerol with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 28.5 kDa (LAP) & 12.8 kDa (Mature), Monomer
SDS-PAGE: 36-42 kDa & 11 kDa, reducing conditions, 80-95 kDa & 24 kDa, non-reducing conditions
Protein length
Leu30-Ser390
Bio-activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 10-70 ng/mL before acid activation and 0.15-0.9 ng/mL after acid activation.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after opening.
3 months, ≤ -20 centigrade under sterile conditions after opening.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 10-70 ng/mL before acid activation and 0.15-0.9 ng/mL after acid activation.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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