Recombinant Human TGFBR1 Protein (Ala25-Glu125), C-hFc-tagged
Product Description
Cat
IMP-9749
Official Symbol
TGFBR1
Product Overview
Recombinant human TGF-beta RI/ALK-5 protein (Ala25-Glu125) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc
Predicted N Terminal
Ala25&Leu34
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 37 kDa (monomer)
SDS-PAGE: 40-60 kDa, reducing conditions
Protein length
Ala25-Glu125
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human TGF-beta RII Fc Chimera is immobilized at 1 μg/mL (100 μL/well), it binds Recombinant Human (rh) TGF-beta RI/ALK-5 Fc Chimera in the presence of rhTGF-beta 1. The concentration of rhTGF-beta RI Fc Chimera that produces 50% of the optimal binding response is approximately 0.05-0.4 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info