Recombinant Human TGFBR1 Protein, C-His&C-hFc-tagged
Product Description
Cat
IMP-1804
Official Symbol
TGFBR1
Product Overview
Recombinant human TGFBR1 (NP_004603.1) extracellular domain (Met 1-Glu 125) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Description
Transforming growth factor, beta receptor I, also known as Transforming growth factor-beta receptor type I, Serine / threonine-protein kinase receptor R4, Activin receptor-like kinase 5, SKR4, ALK-5, and TGFBR1, is a single-pass type I membrane protein that belongs to the protein kinase superfamily and TGFB receptor subfamily. TGFBR1 / ALK-5 is found in all tissues examined. It is most abundant in placenta and least abundant in brain and heart. TGF-beta functions as a tumor suppressor by inhibiting the cell cycle in the G1 phase. Administration of TGF-beta is able to protect against mammary tumor development in transgenic mouse models in vivo. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers, with the majority of colon and gastric cancers being caused by an inactivating mutation of TGF-beta RII. On ligand binding, TGFBR1 / ALK-5 forms a receptor complex consisting of two type I I and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which auto-phosphorylate, then bind and activate SMAD transcriptional regulators. TGF-beta signaling via TGFBR1 / ALK-5 is not required in myocardial cells during mammalian cardiac development, but plays an irreplaceable cell-autonomous role regulating cellular communication, differentiation and proliferation in endocardial and epicardial cells. Defects in TGFBR1 / ALK-5 are the cause of Loeys-Dietz syndrome type 1A (LDS1A), Loeys-Dietz syndrome type 2A (LDS2A), and aortic aneurysm familial thoracic type 5 (AAT5).
Expression System
HEK293
Species
Human
Tag
C-His&C-hFc
Predicted N Terminal
Leu 34
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human TGFBR1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 340 amino acids and has a predicted molecular mass of 38.1 kDa. As a result of glycosylation, the apparent molecular mass of rh TGFBR1/Fc monomer is approximately 45-50 kDa in SDS-PAGE under reducing conditions, with ~10% free Fc fragments.
Protein length
Met1-Glu125
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 85 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized mouse CD105 at 10 μg/mL (100 μL/well) can bind human TGFRB1 with a linear range of 6.4-800 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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